Biogenic Synthesis of Silver Nanoparticles Using Onosma Bourgaei Leaves: Characterization and Antioxidant Activity

Abstract

Green synthesis of nanoparticles has achieved substantial significance recently owing to its application in many fields. The silver nanoparticle synthesis (OB-AgNPs) was accomplished using Onosma bourgaei. Spectroscopic methods were utilized to elucidate the structure of synthesized OB-AgNPs. UV/Vis analysis displayed the absorption at 450 nm. The functional groups were assigned by FTIR. The morphology was presented by SEM and TEM analysis and particles were determined to have a spherical shape. The XRD analysis (2θ) [111, 200, 220, 311, and 222] displayed the face-centered cubic structure of the particle. The particle magnitude of the nanoparticle was established to be 16.3 nm by the Scherrer formula. The zeta potential was defined as -46.1 mV, indicating high stability. Antioxidant activity was conducted using the FRAP, DPPH•, and ABTS•+ assays. OB-AgNPs showed the outstanding ABTS•+ scavenging effect (2.92, IC50) compared with the standard BHT (7.17, IC50, μg/mL).