Detection of Legionella Species in Environmental Water by the Quantitative PCR Method in Combination with Ethidium Monoazide Treatment
Author:
Affiliation:
1. Tsukuba Research Laboratories, Aquas Corporation
2. SCM Center, Takara bio Inc.
Publisher
The Society for Antibacterial and Antifungal Agents, Japan
Subject
Public Health, Environmental and Occupational Health,Applied Microbiology and Biotechnology
Reference12 articles.
1. Chang, B., Sugiyama, K., Taguri, T., Amemura-Maekawa, J., Fumiaki, K., and Watanabe, H. (2009) Specific Detection of Viable Legionella cells by combined use of photoactivated ethidium monoazide PCR/real-time PCR. Appl. Environ. Microbiol., 75, 147-153.
2. Chang, B., Taguri, T., Sugiyama, K., Amemura-Maekawa, J., Fumiaki, K., and Watanabe, H. (2010) Comparison of ethidium monoazide and propidium monoazide for the selective detection of viable Legionella cells. Jpn. J. Infect. Dis., 63, 119-123.
3. Chen, N.T. and Chang, C.W. (2010) Rapid quantification of viable legionellae in water and biofilm using ethidium monoazide coupled with real-time quantitative PCR. J. Appl. Microbiol., 109, 623-634.
4. Delgado-Viscogliosi, P., Solignac, L., and Delattre, J.M. (2009) Viability PCR, a culture-independent method for rapid and selective quantification of viable Legionella pneumophila cells in environmental water samples. Appl. Environ. Microbiol., 75, 3502-3512.
5. Furuhata, K., Annaka, T., Ikedo, M., Fukuyama, M., and Yoshida, S. (2005) Comparison of loop-mediated isothermal amplification (LAMP) and conventional culture for the detection of Legionella species in hot spring water samples in Japan. Biocontrol Sci., 10, 117-120.
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