EVALUATION OF THE ADHESION AND MORPHOLOGY OF STEM CELLS FROM APICAL PAPILLA IN DIRECT CONTACT WITH MTA AND BIODENTINE

Abstract

Purpose: To investigate the use of tricalcium silicate cement Biodentine as an alternative to MTA in regenerative endodontic procedures by assessing the morphology and adhesion of stem cells from apical papilla in direct contact with these materials Materials/Methods: Glass ionomer cement, MTA and Biodentine disks with identical size were created. Stem cells from apical papilla were incubated on these disks, and their size and morphology were assessed with a scanning electron microscope. Results: No adhesion of SCAP was observed when incubating cells with glass ionomer disks, which can be explained by a decrease in pH and a delay in cell proliferation. Cell adhesion was observed in the groups incubated in the presence of MTA and Biodentine discs. Cells adhered to the calcium silicate cements have different morphology and can be round or flat, with several processes. Conclusions: Stem cells from apical papilla adhere to MTA and Biodentiе when in direct contact with discs made of these materials. The cells show different morphology and can be round or flat, with several processes. Calcium silicate cements have biocompatibility and bioactivity and they are suitable materials for the biological treatment of pulp and regenerative endodontics.