Genetic effects of F1 pollen sterility genes S-b, S-d and S-e in rice (Oryza sativa L.)

Abstract

An experimental population commonly used in genetic analyses of gene or quantitative trait loci (QTLs) in rice is chromosome segment substitution lines (CSSLs). In the present study, with the typical indica variety Guangluai 4 as a donor and japonica variety Taichung 65 as a recipient, seven CSSLs carrying F₁pollen sterility genes S-b, S-d, S-e, S-b/S-d, S-b/S-e, S-d/S-e, and S-b/S-d/S-e were obtained by specific selection for the target genes, non-specific selection for the genome of the recurrent parents in four backcross populations (BC₁F₂, BC₂F₂, BC₃F₂ and BC₃F₃). We evaluated the genetic effect of the F₁ pollen sterility genes using 35 F₁hybrid individuals in crosses derived from CSSLs and Taichung 65. Pollen fertility of F₁ hybrid plants was observed and the results indicated that the single genes S-b, S-d and S-e can cause 67.7%, 14.6% and 53.2% of pollen sterility, respectively. Multiple genes S-b/S-d, S-b/S-e, S-d/S-e, and S-b/S-d/S-e can cause 76.6%, 85%, 68.7%, and 93% of pollen sterility, respectively.