Molecular cloning and characterisation of alpha subunit of H+-ATPase in Lactobacillus casei Zhang

Abstract

The acid tolerance is an important property of Lactic acid bacteria as potential probiotics. H⁺-ATPase is considered a key gene in several bacteria with the ability of acid tolerance. We cloned and sequenced the full length cDNA of alpha subunit of H⁺-ATPase gene in <i>Lactobacillus casei</i> Zhang, which had been isolated from traditional home-made koumiss in Inner Mongolia of China. The results showed that the respective cDNA sequence is composed of 1530 nucleotides and codes a putative protein including 509 amino acids. In addition, we also reconstructed the phylogenic trees for H⁺-ATPase gene based on amino acids sequences of diverse strains of Lactic acid bacteria.