FT-IR spectroscopic characteristics of differently cultivated Escherichia coli

Abstract

FT-IR spectra were recorded of <i>Escherichia coli</i> cell mass with the aim of obtaining spectral traits possibly useful in a rapid detection and characterisation of this indicator bacterium. A well differentiated spectrum was obtained from the cell mass harvested in a stationary phase of growth, e.g., after 24 h, from a minimum nutrient broth. The cell mass, harvested either earlier or grown in nutrient solutions which contained an enhanced carbon or nitrogen concentrations delivered somewhat different IR spectra, apparently due to a higher content of nucleic acid components as related to other structural constituents of bacterial cells. Consequently, the FT-IR spectra of <i>E. coli</i>, although rather rapidly to collect, seem only capable of delivering useful and reproducible information if the cell mass is obtained under standardised cultural conditions.