Cryoprotection of porcine cornea: a scanning electron microscopy study
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Published:2012-03-28
Issue:No. 5
Volume:50
Page:219-224
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ISSN:0375-8427
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Container-title:Veterinární Medicína
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language:
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Short-container-title:Veterinarni Medicina
Author:
Neronov A.,Giurov P.,Cholakova M.,Dimitrova M.,Nikolova E.
Abstract
Porcine corneas were frozen with Me<sub>2</sub>SO, glycerol, 1,2-propanediol and PEG-400. The effects of the range of concentrations (5% and 10%) and temperature regimen (1ºC/min and 5ºC/min) were investigated. The integrity of corneal endothelial cells was evaluated by scanning electron microscopy and trypan blue staining. The presence of 5–10% PEG-400 in the protective medium was the most effective in minimizing changes in the integrity of the corneal endothelium during freezing-thawing procedures.
Publisher
Czech Academy of Agricultural Sciences
Subject
General Veterinary
Cited by
1 articles.
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