Are the RNA:DNA ratio and dry-weight-at-length suitable growth proxies for brown shrimps (Crangon crangon)?

Abstract

Growth rates are of fundamental interest for studying population dynamics of species when no age information is available. In these cases in situ growth proxies must be identified and validated, and here we tested whether RNA-DNA ratio (RD) and dry weight condition (DWC) can act as such proxies for the shrimp Crangon crangon. Growth rates (mm d–1) were determined for male and female shrimps (20 to 67 mm) reared at five different temperatures (5°C to 25°C) and caught in two different months (May, July). Due to the potential interactive effects of the factors sex, size, temperature and time, different statistical analysis were applied to test for linear interactions (factorial analysis of mixed data, FAMDs), nonlinear additive effects (generalized additive models, GAMs) and nonlinear interactions (nonlinear regression combined with GAMs). The FAMD indicated positive correlations between length, month and growth rate. A positive correlation between DWC and sex and between RD and temperature was also indicated. RD was not significant in the GAM but a nonlinear link between length, temperature and growth was found. Finally, an extended Bertalanffy growth model was fitted to sex-specific growth rates and in a second step a GAM was fitted to the differences (residuals) between observed and predicted growth. RD again had no significant explanatory power for growth and, although it is a commonly used growth proxy, we concluded that it is not a suitable index for evaluating length growth of C. crangon.