EFFECT OF 2-HYDROXY-N-NAPHTHALENE-1-YL-2-(2-OXO-1,2-DIHYDRO-INDOLE-3-YLYDENE)-ACETAMIDE ON KIDNEY FUNCTION IN EXPERIMENTAL RENAL FAILURE

Abstract

Heterocyclic compounds are the basis for a significant number of medicinal substances. Among them, there is indole and derivatives of 2-oxoindolin-3-glyoxylic acid. This group of compounds has been found out to have neurotropic effects as well as cardio- and hepatoprotective properties. This preceded the study of their nephroprotective activity in the experimental pathology. The aim of the work is to study the effect of 2-hydroxy-N-naphthalen-1-yl-2(2-oxo-1,2-dihydro-indol-3-ylidene)-acetamide on the excretory function of kidneys in the experimental acute renal failure. Materials and methods. Experiments were performed on 24 adult male rats. Compound 18 was administered is a dose of 12 mg/kg. Ethyl-methyl-hydroxypyridine succinate in a dose of 100 mg/kg served as a reference preparation. Glycerol-induced acute kidney damage was modelled by intramuscular injection of the 50 % glycerol solution. Under the condition of water load, the excretory kidney function, kidney mass coefficients, creatinine and urea content in the blood plasma were assessed by the standard methods. Results. Glycerol-induced kidney damage did not cause the death of test animals. It significantly reduced the rate of glomerular filtration and water resorption; proteinuria developed due to an increase in protein concentration and a decrease in its excretion as compared to the parameters of intact rats. This change in the kidney function was accompanied by an increase in the content of creatinine and urea in the blood plasma. The use of the reference preparation ethyl methyl hydroxypyridine succinate prevented a decrease in the rate of glomerular filtration, increased creatinine excretion, but did not affect the values ​​of other parameters. Compound 18 showed an antiproteinuric effect. The substance under investigation did not change probably or worsen other parameters. In glycerol-induced kidney damage, ethyl methyl hydroxypyridine succinate and compound 18 did not effectively prevent the increase in kidney mass coefficient. Conclusion. In glycerol-induced kidney damage, compound 18 in a dose of 12 mg/kg did not cause a pronounced nephroprotective effect.