Author:
Semenovykh P.,Nesen A.,Savicheva K.,Schenyavska O.,Shkapo V.
Abstract
The aim of this work is to study the state of the prooxidative-antioxidant balance in patients with diabetic nephropathy depending on the functional state of the kidneys and taking into account the polymorphism of ACE gene. Participants and methods. This study involved 82 patients with type 2 diabetic and nephropathy. The control group consisted of 20 healthy individuals. The prooxidant-antioxidant balance of blood serum was calculated as the ratio of total hydroperoxide content to total antioxidant activity. The content of total hydroperoxides and total antioxidant activity were determined by the colorimetric method. Results. Oxidative stress plays an important role in the pathogenesis of diabetic nephropathy. Angiotensin I-converting enzyme (ACE) gene polymorphism is associated with the development and progression of acute and chronic kidney diseases. However, the features of antioxidant protection in patients with diabetic nephropathy, who are carriers of different genotypes of this polymorphism, have not been studied. Association analysis using the SNPStats online program has demonstrated that in patients with diabetic nephropathy, the I/I polymorphism is associated with an increase in the concentration of total hydroperoxides in blood serum (difference = 23.62 (6.16-41.08); p = 0.017 — codominant model inheritance; difference = 22.73 (7.70-37.75); p = 0.0042 — recessive model of inheritance). We found a significant increase of total hydroperoxides in diabetic nephropathy patients - carriers of the homozygous I/I genotype of the ACE gene polymorphism, compared to carriers of the D-allele (I/D and D/D genotypes), p=0.03.The highest values of prooxidant-antioxidant balance of blood serum in patients with diabetic nephropathy are noted in carriers of the I/I and I/D genotypes of the ACE gene/ These findings may indicate a negative effect of the I allele on the general state of the prooxidant-antioxidant balance in this category of patients. Conclusions. The analysis of the data we obtained allows us to assume an important influence of the ACE gene polymorphism on the pathogenesis of diabetic kidney disease due to changes in the prooxidant-antioxidant balance in patients with type 2 diabetes.
Publisher
Ukrainian Medical Stomatological Academy
Subject
General Materials Science
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