THE IMPACT OF CENTRAL DEPRIVATION OF TESTOSTERONE ON THE STRUCTURE OF RED BONE MARROW IN MONOCYTOPOESIS IN THE EARLY TERMS OF THE EXPERIMENT

Abstract

The effect of androgen deficiency on haematopoiesis has not been sufficiently studied yet. It is known that androgens have a double effect on the hematopoietic system. Hematopoietic tissue is a continuously regenerating dynamic system; therefore, the issues of hematopoietic kinetics and its regulation are key ones to understand the pathogenesis of blood diseases in general. It has been determined that the development of all hematopoietic cells results from the proliferation and differentiation of a single pluripotent blood stem cell under the influence of surrounding cells. The effect of prolonged central deprivation of testosterone synthesis on morphological changes in hematopoietic organs is still not enough described in the scientific literature. The aim of this study is to evaluate the microscopic structure of hematopoietic cells in rats during the central deprivation of testosterone synthesis by diferelin injection on the 30 day of the experiment. Materials and methods. The experiment was performed on 20 adult male white rats divided into 2 groups of 10 animals in each group: control and test group. The animals in the experimental group were injected with diferelin (Triptorelin embonate) subcutaneously in a dose of 0.3 mg of active substance per kg / body wt. The rats in the control group received a saline injection. The experiment lasted 30 days. Results. The morphological study of the red bone marrow in the test group of animals on the 30 day of the experiment has demonstrated the structure of the organ is represented by two components: stromal and parenchymal. The stroma consists of reticular cells forming a network of reticular fibbers, most of which are threaded with sinusoidal capillaries. In the middle of the capillaries there is a dense arrangement of erythrocytes in the form of a "column of coins". The pericapillary space is filled with hematopoietic cells at different stages of differentiation. The monoblastic clone is represented by monocyte cells, promonocytes, monoblasts, and progenitor cells that are of the same mophrological structure and structure under light microscopy.