EFFECT OF MODULATORS OF REDOX-SENSITIVE TRANSCRIPTIONAL TRANSCRIPTION FACTORS ON MORPHOLOGICAL CHANGES IN PERIODONTIUM OF RATS DURING SYSTEM INFLAMMATORY RESPONSE

Abstract

The experiment carried out on 30 white rats was aimed at studying pathomorphological changes in periodontal tissues during lipopolysaccharide-induced systemic inflammatory response and their nature when a water-soluble form of quercetin and epigallocatechin-3-gallate was administered to the animals. The rats were divided into 4 groups: the 1st group included animals after systemic administration of S. typhi lipopolysaccharide (pyrogenal); the 2nd and 3rd group involved animals, which were injected with a water-soluble form of quercetin (10 mg/kg) and epigallocatechin-3-gallate (21.1 mg/kg) intraperitoneally (every 3 days) starting from the 30th day of the experiment. Pyrogenal was administered in a dose of 0.4 μg/kg 3 times during the 1st week and once a week for the next 7 weeks. Sections of 4–6 μm thick were cut from paraffin blocks and stained with hematoxylin and eosin. It has been found out that experimental modeling of a systemic inflammatory response leads to the development of morphological signs of periodontitis, which are manifested by inflammation and destruction of periodontal pockets, the formation of pathological periodontal pockets, destructive changes in bone tissues of the alveolar process and in the cementum of the dental root. The use of the water-soluble form of quercetin and epigallocatechin-3-gallate in the experimental conditions changes the morphological picture of inflammatory and destructive processes in the periodontium: it prevents the formation of purulent exudate, reduces the intensity of the resorption of cementum and bone tissue of the alveolar process, accelerates the development and maturation of granulation tissue in the walls of periodontium, promotes the replacement of bone defects with connective tissue, and enhances the regeneration of cementum.