CHARACTERISTICS OF ROOT CANAL MICROBIOTA IN ACUTE PULPITIS OF TEMPORARY TEETH

Abstract

Aims. To determine the representatives of the microbiota of the root canals seeded in acute forms of pulpitis of temporary teeth among children of the Transcarpathian region, taking into account the endemic features of the Transcarpathian region and its influence on the course of the carious process. Materials and methods. Collection of biomaterial was carried out using transport tubes with Amies transport medium. The biomaterial was delivered to the laboratory within 2-4 hours after selection. In order to evaluate the microbiota of the root canals, cultivation of biomaterial was carried out on differential diagnostic nutrient media. The material was sown by the method of sector sowing according to Gold: Saburo for the cultivation of microscopic mushrooms; blood agar (MPA + 5% blood) Streptococcus and Neisseria bacteria; bacteria of the family Enterobacteriaceae Endo and Levin; yolk-salt agar with mannitol bacteria of the genus Staphylococcus; enterococcus agar for isolation of enterococci (Farmaktiv, Ukraine). Additionally, the biomaterial was sown on a chromogenic medium (bioMerieux, France). Microorganisms were identified by cultural, morphological and biochemical characteristics using API-test systems (bioMerieux). The results of the quantitative and qualitative evaluation of the microbiota of the oral cavity were expressed in CFU/ml. Results and discussion. Today, in the daily practice of a dentist, among issues of diagnosis, prevention and treatment of dental diseases, the study of the microbiota of the oral cavity, which is a collection of different taxonomic groups of microbes that inhabit the oral cavity and enter into biochemical, immunological and other relationships with macroorganism and each other. At the same time, the microbial landscape of the oral cavity is represented by two types of microbiota: obligate, that is, one that is constantly present in the oral cavity and is mainly saprophytic, and provides metabolic processes and the function of protecting the body from virulent infectious agents, and facultative, which is represented opportunistic microorganisms, which, when immune protection is reduced, acquire aggressive properties and contribute to the development of diseases [1]. Conclusion. Opportunistic bacteria play a significant role in the development of inflammatory diseases. Considering the fact that pulpitis is an inflammatory reaction caused by the activity of opportunistic microflora, the study of the characteristics of the microbiota of root canals is relevant, which will help to improve the selection of antiseptic agents for medicinal treatment of root canals in order to obtain positive delayed treatment results.