ANALYSIS OF ANTIOXIDANT SYSTEM OF TEST ANIMALS IN THE DYNAMICS OF THE WOUND PROCESS AT LOCAL APPLICATION OF HYDROGEL DRESSINGS

Abstract

Introduction. The development of the inflammatory process is always accompanied by the activation of lipid peroxidation, which is caused by tissue hypoxia. This, in turn, catalyzes a change in the physiological parameters of the antioxidant system. The aim of the study is to analyze the dynamics of the antioxidant balance of wounds in an experiment using hydrogels saturated with silver ions and an antioxidant drug for the local treatment of inflammatory processes. Object and research methods. The experimental study was carried out on 80 Wistar rats weighing 150 to 200 grams. Animals were removed from the experiment. for 3, 7, 10, 14 days. Catalase activity in the dynamics of the wound process was determined. Research results and their discussion. The content of catalase on the 7th day in the third experimental group is 0.51±0.03 μKat/l. On the 10th day, the indicator of the antioxidant system in the second (0.44±0.05 μKat/l) and third experimental groups was 0.42±0.02 μKat/l. At the final stage of the study on the 14th day, the analysis of catalase content showed that in all animals with a simulated inflammatory process, the indicator of antioxidant activity had no significant differences between themselves and, importantly, with the indicators of intact animals (0.33±0.01 μKat /l). The indicators of the control (0.33±0.04 μKat/l) and experimental groups were as follows: the first experimental group – 0.32±0.06, the second experimental group – 0.36±0.04, the third experimental group – 0, 38±0.06 μKat/l. Antioxidant protection was supplemented by local release of a drug with antioxidant action from the hydrogels, which contributed to a reduction in the intensity of free radical oxidation processes. That is, in almost all groups of animals, which were simulated purulent-inflammatory wounds, indicators of catalase activity approached the level of intact animals already on the 14th day. Conclusions. Application for local treatment of hydrogel bandages saturated with an antioxidant drug, which is released into the wound surface for a long time, promotes the activation of antioxidant protection and suppresses the processes of free radical formation.