DYNAMICS OF BONE TISSUE REMODELLING MARKERS IN BIOLOGICAL FLUIDS OF PATIENTS WITH GENERALISED PERIODONTITIS ASSOCIATED WITH BLOOD GROUP AFFILIATION

Abstract

Despite the large number of studies on the dynamics of bone remodeling markers in blood and oral fluid in patients with generalized periodontitis (GP), there is little information in the literature on the relationship between periodontal disease and such a genetically determined trait as blood group. Therefore, the identification of group-specific features may play an important role in the development of pathological processes in the periodontium, which is planned to be analyzed in this article. The aim of the study – to analyze the dynamics of bone remodeling markers in biological fluids of patients with generalized periodontitis associated with blood group. Materials and Methods. Laboratory studies were performed in 124 patients with generalized periodontitis (main group) and 120 healthy individuals (comparison group) with different blood group. The activity of tartrate-resistant acid phosphatase was determined by enzyme-linked immunosorbent assay using the Bone TRAP Assay kit. C-terminal telopeptides of human collagen type I and osteocalcin in blood serum and oral fluid were determined by enzyme-linked immunosorbent assay using the N-MID-osteocalcin and Crosslaps™ ELISA test systems from Nordic Bioscience Diagnostics A/S. The activity of the bone enzyme alkaline phosphatase was determined by the method of enzyme-linked immunosorbent assay using standard kits from «Filisit-Diagnostics», Ukraine. Results and Discussion. As a result of the studies, it was found that in the subjects of the main group, an increase in the activity of bone resorption markers was determined in the blood: TRACP – by 36,68 %, P1CP – by 15,23 % against the background of a decrease in the activity of bone formation markers: osteocalcin by 22,95 %, BSALP – by 30,66 % compared to the corresponding data in patients with intact periodontium, p<0,01. It was noteworthy that bone formation, according to the content of BSALP and OC in the blood serum, was most impaired in carriers of 0 (I) and B (III) blood groups with HP. At the same time, in representatives of A (II) and 0 (I) blood groups with GP, bone resorption processes prevailed according to the data of TRACP and P1CP markers in the blood serum. The imbalance in the values of bone remodeling markers in the oral fluid of patients with GP was accompanied by an increase in TRACP activity by 23,02 %, P1CP – by 1,9 times, against a decrease in BSALP activity by 18,95 % and osteocalcin – by 47,57 % compared with the data in the subjects of the comparison group, p<0,01. Conclusions. Thus, a significant imbalance in markers of bone remodeling was found in patients with generalized periodontitis, regardless of blood group. The greatest imbalance of these markers in the blood and oral fluid was determined in patients with generalized periodontitis of blood group 0 (I) and A (II) carriers by a decrease in osteocalcin levels against the background of increased content of resistant alkaline phosphatase tartrate and C-terminal human collagen type 1 telopeptide in biological fluids.