Abstract
Suture material used in abdominal surgery must have more or less antigenic properties so that during wound healing it could be destroyed by an immune response to rejection, which is known to involve cytotoxic T-lymphocytes and macrophages. This, in turn, can lead to the development of aseptic peritonitis. Therefore, while planning an experimental simulation of aseptic peritonitis, we decided to use catgut thread as an antigenic stimulation of the local immune system. The aim of our research was to study the morphological criteria of the microscopic structure and tinctorial properties of catgut thread. The material of the research was the sterile catgut thread sized 2/0, 0.3 mm thick, of equal lengths, which we twisted arbitrarily into flat compact globules occupying an area of approximately 1 cm2. In order to carry out a histological examination, some catgut globules were subjected to total staining with a hematoxylineosin solution and subsequent embedding of these globules in a paraffin block and making appropriate sections from it. The other half of the catgut globules were studied using the method of epoxy plastination with the production of grinds with staining by a 1% solution of methylene blue on a 1% borax and without any staining. After the total staining of the catgut globules with a hematoxylin-eosin solution, a clear acidophilic reaction was revealed, which was manifested in the intense pink color. Catgut thread has a non-uniform fibrous structure, in which dark streaks stand out, dividing it into a series of longitudinal, lighter, layered fascicles, which consist of a dense collection of thin fibrillar elements. These fibrillar elements are orderly fascicles of collagen fibers, while the dark streaks are layers of loose fibrous connective tissue. These structures can objectively be indicative of morphological criteria in the microscopic analysis of destructive changes of a catgut implant in the peritoneal cavity in an experimental animal.
Publisher
Ukrainian Medical Stomatological Academy
Reference18 articles.
1. Titley-Diaz WH, De Cicco FL. Suture Hypersensitivity. 2022 Sep 18. In: StatPearls [Internet]. Treasure Island (FL): StatPearls Publishing; 2022 Jan. PMID: 32965959.
2. Lock AM, Gao R, Naot D, Coleman B, Cornish J, Musson DS. Induction of immune gene expression and inflammatory mediator release by commonly used surgical suture materials: an experimental in vitro study. Patient Saf Surg. 2017 May 31;11:16. doi: 10.1186/s13037-0170132-2.
3. Lovric V, Goldberg MJ, Heuberer PR, Oliver RA, Stone D, Laky B, Page RS, Walsh WR. Suture wear particles cause a significant inflammatory response in a murine synovial airpouch model. J Orthop Surg Res. 2018 Dec 6;13(1):311. doi: 10.1186/s13018-018-1026-4.
4. Bilash SM, Pronina OM, Sуdorenko MI, Kobenyak MM. Problemy vyboru shovnoho materialu pry operatsiyakh na kyshechnyku. Bulletin of problems biology and medicine. 2018;1(2 (144)):13-6 DOI: 10.29254/2077-4214-2018-2144-13-16.
5. Kostylenko YuP, Hryn VH, Hryn KV, Harbolynska LM. Morfolohichni osnovy imunnoyi systemy. Lviv: «Mahnoliya – 2006»; 2022. 136s. ISBN 978-617-574-237-2/