Affiliation:
1. Institute of Bioorganic Chemistry of the National Academy of Sciences of Belarus
Abstract
Growth hormone (GH) is a polypeptide produced in the anterior pituitary lobe that triggers different biochemical pathways and increases cell proliferation and growth. In this work, the pNic based vector for periplasmatic secretion was developed. Recombinant human growth hormone was produced via periplasmatic secretion (42h, 30 °С) followed by several steps of purification. GH obtained does not contain His-tag. Expression level of 2,85 mg per1 literof bacterial culture was achieved.
Publisher
Publishing House Belorusskaya Nauka
Subject
Inorganic Chemistry,Organic Chemistry,Chemistry (miscellaneous),Analytical Chemistry