Optimization of the technology for obtaining guide RNA using plate automatic synthesizer-Reference-Cited by-同舟云学术

Optimization of the technology for obtaining guide RNA using plate automatic synthesizer

Published:2022-11-29 Issue:4 Volume:58 Page:398-406
ISSN:2524-2342
Container-title:Proceedings of the National Academy of Sciences of Belarus, Chemical Series
language:
Short-container-title:Vescì Akademìì navuk Belarusì. Seryâ himičnyh navuk

Author:

Ulashchik E. A.¹,Akhlamionok T. P.¹,Baryshchyk P. Y.¹,Sharko O. L.¹,Shmanai V. V.²

Affiliation:

1. Shmanai Institute of Physical Organic Chemistry, National Academy of Sciences of Belarus

2. Institute of Physical Organic Chemistry, National Academy of Sciences of Belarus

Abstract

A synthetic protocol was developed and optimal reagents have been selected for obtaining guide RNA oligonucleotides for the CRISPR/Cas system using ASM-2000 automatic synthesizer in 500 nmol scale. Methods for the isolation, purification and analytical control of synthetic RNA oligonucleotides have been developed. The improved technology has been used for preparation of guide RNAs for the CRISPR Cas12a system.

Publisher

Publishing House Belorusskaya Nauka

Subject

Inorganic Chemistry,Organic Chemistry,Chemistry (miscellaneous),Analytical Chemistry

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