Registration of entomopatogenic micromycete <i>Cordyceps</i> sp. in bloodsucking blackflies <i>Wilhelmia equine</i> (Linnaeus, 1758)-Reference-Cited by-同舟云学术

Registration of entomopatogenic micromycete Cordyceps sp. in bloodsucking blackflies Wilhelmia equine (Linnaeus, 1758)

Published:2020-02-11 Issue:1 Volume:65 Page:43-49
ISSN:2524-230X
Container-title:Proceedings of the National Academy of Sciences of Belarus, Biological Series
language:
Short-container-title:Vescì Akademìì navuk Belarusì. Seryâ biâlagičnyh navuk

Author:

Dovnar D. V.¹,Yankovskaya E. N.²,Voitka D. V.²,Baranov O. Yu.³,Kaplich V. M.⁴,Kanterova H. V.⁵

Affiliation:

1. Scientific and Practical Center for Bioresources of the National Academy of Sciences of Belarus

2. Institute of Plant Protection of the National Academy of Sciences of Belarus

3. Forest Institute of the National Academy of Sciences of Belarus

4. Belarusian State Technological University

5. Institute of Microbiology of the National Academy of Sciences of Belarus

Abstract

An entomopathogenic fungi belonging to the genus Cordyceps was isolated from the affected larvae of the bloodsucking blackflies Wilhelmia equina (Linnaeus, 1758). Larval stage of Simulium were collected on the territory of the Lepel district of the Vitebsk region in the river Essa in July 2018. Isolate was identified using cultural-morphological and molecular-genetic methods.In vitro experiments comparative evaluation of biological activity of isolated strain Cordyceps sp. and Beauveria bassiana 10-06 was carried out for 3rd instar larvae of W. equina. Two different concentrations 1∙106 and 1∙107 conidia/ml were made and tested in 3 replications. On the 3rd day of incubation both fungi caused a significant mortality of test-objects (p = 0.03) compared to control. The biological efficiency of the concentration of 107 соnidia/ml of the strain Beauveria bassiana 10-06 on the 3rd day was 94.4 %, on the 4th – 98.3 %, of the strain Cordyceps sp. – 76.0 and 88.9 % respectively.

Publisher

Publishing House Belorusskaya Nauka

Subject

General Earth and Planetary Sciences,General Environmental Science

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