Riboflavin-photosensitized thiamine oxidation in aqueous solutions on exposure to ultraviolet and visible light-Reference-Cited by-同舟云学术

Riboflavin-photosensitized thiamine oxidation in aqueous solutions on exposure to ultraviolet and visible light

Published:2020-05-16 Issue:2 Volume:65 Page:199-211
ISSN:2524-230X
Container-title:Proceedings of the National Academy of Sciences of Belarus, Biological Series
language:
Short-container-title:Vescì Akademìì navuk Belarusì. Seryâ biâlagičnyh navuk

Author:

Stepuro I. I.¹,Labor S. A.¹,Stsiapura V. I.²,Yantsevich A. V.³

Affiliation:

1. Institute of Biochemistry of Biologically Active Compounds of the National Academy of Sciences of Belarus

2. Yanka Kupala State University of Grodno

3. Institute of Bioorganic Chemistry of the National Academy of Sciences of Belarus

Abstract

It was shown the irradiation of aqueous solutions of thiamine and riboflavin with ultraviolet UVA (315–400 nm) and visible light (λ > 400 nm) leads to oxidation of thiamine to thiochrome and oxodihydrothiochrome. In absence of riboflavin in aerobic conditions thiamine oxidation to thiochrome was mainly due to reaction with singlet oxygen. Under anaerobic conditions, thiamine in the presence of riboflavin was photostable and UV and visible light irradiation of their aqueous solutions did not result in thiamine oxidation. Under aerobic conditions, thiochrome was oxidized to form oxodihydrothiohrome in reactions with singlet oxygen and riboflavin in triplet states. Under anaerobic conditions, oxidation of thiochrome to oxodihydrothiochrome occurred due to reaction with riboflavin in triplet state. Possible mechanisms of the damage of cellular eye structures due to inactivation of thiamine-dependent enzymes on exposure to intensive UVA and visible light in the presence of endogenous photosensitizer riboflavin are discussed.

Publisher

Publishing House Belorusskaya Nauka

Subject

General Earth and Planetary Sciences,General Environmental Science

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