EHBP1 is critically involved in dendritic arbor formation and is coupled to factors promoting actin filament formation

Abstract

The coordinated action of a plethora of factors is required for organization and dynamics of membranous structures critically underlying development and function of cells, organs and organisms. The evolutionary acquisition of additional amino acid motifs allows for expansion and/or specification of protein functions. We identify a thus far unrecognized motif specific for chordata EHBP1 proteins and demonstrate that this motif is critically required for interaction with syndapin I, an F-BAR domain-containing, membrane-shaping protein predominantly expressed in neurons. Gain-of-function and loss-of-function studies in rat primary hippocampal neurons (of mixed sexes) unraveled that EHBP1 has an important role in neuromorphogenesis. Surprisingly, our analyses uncovered that this newly identified function of EHBP1 did not require the domain responsible for Rab GTPase binding but was strictly dependent on EHBP1's syndapin I binding interface and on the presence of syndapin I in the developing neurons. These findings were underscored by temporally and spatially remarkable overlapping dynamics of EHBP1 and syndapin I at nascent dendritic branch sites. In addition, rescue experiments demonstrated a necessity of two additional EHBP1 domains for dendritic arborization, the C2 and the CH domain. Importantly, the additionally uncovered critical involvement of the actin nucleator Cobl in EHBP1 functions suggested that not only static association with F-actin via EHBP1's CH domain is important for dendritic arbor formation but also actin nucleation. Syndapin interactions organize ternary protein complexes composed of EHBP1, syndapin I and Cobl and our functional data show that only together these factors give rise to proper cell shape during neuronal development.Significance StatementThe development and function of cells, organs and organisms requires proper organization and dynamics of membranous structures dependent on the coordinated action of a plethora of factors. We here identify a motif specific for EHBP1 proteins from chordates that is critically required for interaction with syndapin I, a neuronal membrane-shaping protein. Functional studies unraveled a thus far unrecognized important role of in dendritic arbor formation of developing neurons and that this function strictly depends on syndapin I. EHBP1 accumulated together with syndapin I, the actin nucleator Cobl and F-actin at nascent dendritic branch sites. Syndapin interactions organize ternary EHBP1/syndapin I/Cobl complexes and functional examinations reveal that only together these factors give rise to proper cell shape during neuronal development.