Macrophage Responses Against Acellularized Dermal Matrix from Human Versus Porcine Predicts the In-vivo Fibrosis

Abstract

Background: Due to the possible difference in the manufacturer’s processes, in-vitro and in-vivo tests might be beneficial for the selection of an Acellularized Dermal Matrix (ADM). Methods: ADMs from human and porcine were replaced the mouse skins and tested macrophage reactions using RAW264.7 cells. Results: In comparison with porcine ADM, human ADM induced higher reaction and fibrosis on mouse histology at 2 wk (but became similar between groups at 14 wk post-operation) and induced higher upregulation of pro-inflammatory genes at 24 h post-macrophage stimulation. Conclusion: Macrophage responses were proposed to use for the prediction of in-vivo ADM responses which might be beneficial for the ADM selection.