Abstract
The plasmid vector pET-28b(+) named “Young” was produced according a work of 2014
(Ref.1). This construct is designed to allow the expression of a 13.6 kDA protein with a Cterminal 6 histag. It is supposed to be an anti-HRP (Horse-Radish Peroxydase protein). This protein was not expressed in first E.coli we attempt to explain this phenomenon.
Publisher
Athenaeum Scientific Publishers
Subject
General Medicine,General Computer Science,Psychiatry and Mental health,Neuropsychology and Physiological Psychology,Automotive Engineering,General Medicine,General Arts and Humanities,General Engineering,General Earth and Planetary Sciences,General Environmental Science,General Medicine,General Economics, Econometrics and Finance