Optical clearing imaging assisted evaluation of urokinase thrombolytic therapy on cerebral vessels with different sizes

Abstract

Ischemic stroke is caused by occlusion of the blood vessels in the brain, where intravenous thrombolytic therapy is the most effective treatment. Urokinase is a commonly used drug for intravenous thrombolytic therapy, while the effect of vessel size has not been thoroughly studied on urokinase. In this work, using the thrombin-combined photothrombosis model and craniotomy-free skull optical clearing window, we studied the recanalization of different cortical vessels after urokinase treatment. The results demonstrated that, compared to small vessels in distal middle cerebral artery (MCA) and large MCA, urokinase has the best therapeutic effect on secondary branches of MCA. This study holds potential to provide references for the clinical applications of urokinase.