Quantitative phase imaging based on motionless optical scanning holography

Abstract

Optical scanning holography (OSH) can be applied to 3D fluorescent imaging. However, the optical setup for OSH is complicated due to the requirement of a phase shifter, a 2D mechanical scanner, and an interferometer. Although motionless optical scanning holography (MOSH) can overcome the problem, quantitative phase imaging (QPI) has not yet been realized because MOSH can only obtain incoherent holograms. If QPI in MOSH is realized, MOSH can be applied to various applications. In this Letter, MOSH-based QPI (MOSH-QPI) is proposed. In addition, a simple description of a coherent mode of OSH is presented. In the proof-of-principle experiment, the spatially divided phase-shifting technique is applied to reduce the number of measurements. The feasibility of MOSH-QPI is confirmed by measuring a phase distribution of a microlens array. MOSH-QPI is also applied to measure practical samples, and its results are compared with the experimental results of the conventional one using a Mach–Zehnder interferometer.