Affiliation:
1. State Museum of Natural History
2. Karlsruhe Institute of Technology (KIT)
3. Heidelberg University
4. European X-Ray Free-Electron Laser Facility GmbH
5. Julius Kühn-Institute
Abstract
X-ray imaging enables the study of morphodynamic and physiological
processes in living organisms. However, the required photon flux
increases with the desired spatial resolution and with it the
requirements for dose efficiency. We realize full-field imaging at
micrometer resolution close to the highest possible dose efficiency.
This is achieved by combining propagation-based phase contrast with
Bragg crystal optics and a high-Z single-photon-counting detector, all
designed for X-ray energies that allow minimal dose for a given image
quality. We prove the superior imaging performance compared to
conventional systems and, in particular, show a substantial increase
in dose efficiency for high spatial frequencies that comprise the
relevant high-resolution components of the image. We demonstrate the
potential of the technique by a behavioral in
vivo study of submillimeter-sized parasitoid chalcid wasps
within their host eggs before and during emergence. The findings show
that the technique opens up new possibilities for dose-sensitive
studies at micrometer resolution, not only in life sciences but also
in materials research.
Funder
Bundesministerium für Bildung und
Forschung
Subject
Atomic and Molecular Physics, and Optics,Electronic, Optical and Magnetic Materials
Cited by
1 articles.
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