Analysis of SARS-CoV-2 spike RBD binding to ACE2 and its inhibition by fungal cohaerin C using surface enhanced Raman spectroscopy-Reference-Cited by-同舟云学术

Analysis of SARS-CoV-2 spike RBD binding to ACE2 and its inhibition by fungal cohaerin C using surface enhanced Raman spectroscopy

Published:2023-07-12 Issue:8 Volume:14 Page:4097
ISSN:2156-7085
Container-title:Biomedical Optics Express
language:en
Short-container-title:Biomed. Opt. Express

Author:

Wetzel Christoph^ORCID,Jansen-Olliges Linda¹^ORCID,Stadler Marc²³,Surup Frank²³,Zeilinger Carsten¹^ORCID,Roth Bernhard¹^ORCID

Affiliation:

1. Leibniz University Hannover

2. Helmholtz Centre for Infection Research GmbH

3. Technische Universität Braunschweig, Institute of Microbiology

Abstract

The structure of the SARS-CoV-2 spike RBD and human ACE2 as well as changes in the structure due to binding activities were analysed using surface enhanced Raman spectroscopy. The inhibitor cohaerin C was applied to inhibit the binding between spike RBD and ACE2. Differences and changes in the Raman spectra were determined using deconvolution of the amide bands and principal component analysis. We thus demonstrate a fast and label-free analysis of the protein structures and the differentiation between bound and unbound states. The approach is suitable for sensing and screening and might be relevant to investigate other protein systems as well.

Funder

Deutsche Forschungsgemeinschaft

Bundesministerium für Bildung und Forschung

Publisher

Optica Publishing Group

Subject

Atomic and Molecular Physics, and Optics,Biotechnology

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