Adaptive optics for optical microscopy [Invited]

Author:

Zhang Qinrong,Hu Qi1,Berlage Caroline23ORCID,Kner Peter4ORCID,Judkewitz Benjamin2ORCID,Booth Martin1ORCID,Ji NaORCID

Affiliation:

1. University of Oxford

2. Charité - Universitätsmedizin Berlin

3. Institute for Biology

4. University of Georgia

Abstract

Optical microscopy is widely used to visualize fine structures. When applied to bioimaging, its performance is often degraded by sample-induced aberrations. In recent years, adaptive optics (AO), originally developed to correct for atmosphere-associated aberrations, has been applied to a wide range of microscopy modalities, enabling high- or super-resolution imaging of biological structure and function in complex tissues. Here, we review classic and recently developed AO techniques and their applications in optical microscopy.

Funder

U.S. Department of Energy

European Research Council

National Institutes of Health

Boehringer Ingelheim Fonds

Wellcome Trust

Publisher

Optica Publishing Group

Subject

Atomic and Molecular Physics, and Optics,Biotechnology

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