Affiliation:
1. The First Affiliated Hospital of Xi’an Jiaotong University
2. Xi’an Jiaotong University
Abstract
Misfolding of amyloid protein will cause neurodegeneration and trigger conformational disease. The lack of an effective detection approach is a brake on unveiling the mechanism of protein misfolding. We theoretically proposed a novel metasurface-based biosensor for characterizing the protein’s conformation. The coupling complementary split ring resonator (cSRR) was engineered to manipulate incident waves in the near-infrared (NIR) and mid-infrared (MIR) windows at the same sensing surface. The cSRRs had the advantages of intensifying the electric field and sharpening the resonance profile, resulting in a highly qualified biosensing performance. In the NIR window, the biolayer’s refractive index and thickness change were detected by the dual-wavelength, which resolved into an optogeometrical parameter of the amyloid biolayer. In the MIR window, the resonant wave specifically triggered the rotation-vibration transition of amyloid protein molecules with different conformations, which was shown as the unique Amide I and II bands in the fingerprint spectrum. Thus, our proposed biosensor presented sensitive detection of biolayer and specific identification of constituent molecules. It is helpful to interpret the protein’s misfolding behavior on the molecular level by associating the biolayer’s structure and the constituent molecule’s conformational change.
Funder
China Scholarship Council
Talent service enterprise projects in colleges and universities
Natural Science Foundation of Shaanxi Province
National Natural Science Foundation of China
Subject
Atomic and Molecular Physics, and Optics