Affiliation:
1. Dongguan University of Technology
2. Shantou University
3. Shantou University Medical College Cancer Hospital
Abstract
The detection of circulating tumor cells (CTCs) still faces a huge challenge partially because of low abundance of CTCs (1-10 cells/mL). In this work, a plasmonic titled fiber Bragg grating biosensor is proposed for detection of breast cancer cells. The biosensor is made by an 18° TFBG with a 50 nm-thick gold nanofilm coating over the surface of the fiber, further immobilized with a specific antibody against GPR30, which is a membrane receptor expressed in many breast cancers, serving as bait. In vitro tests have confirmed that the proposed biosensor can detect breast cancer cells in concentration of 5 cells/mL within 20 minutes and has good linearity in the range of 5–1000 cells/mL, which has met the requirement of CTC detection in real conditions. Furthermore, theoretical analysis based on the experimental results shows that the limit of detection can even reach single-cell level. Our proposed biosensor has a simple structure, is easy to manufacture, is of small size, and has a good performance, making it a good choice for real-time, label-free, and milliliter-volume detection of cancer cells in future.
Funder
Basic and Applied Basic Research Foundation of Guangdong Province
2020 LKSF Cross-Disciplinary Research Projects
Key Project of Basic Research and Applied Basic Research in Ordinary Universities of Guangdong Province
Subject
Atomic and Molecular Physics, and Optics,Biotechnology
Cited by
6 articles.
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