Label-free detection of breast cancer cells using a functionalized tilted fiber grating

Author:

Chen Xiaoyong1,Xu Pin2,Lin Wenwei2,Jiang Jin3,Qu Hang2,Hu Xuehao2ORCID,Sun Jinghua1ORCID,Cui Yukun3

Affiliation:

1. Dongguan University of Technology

2. Shantou University

3. Shantou University Medical College Cancer Hospital

Abstract

The detection of circulating tumor cells (CTCs) still faces a huge challenge partially because of low abundance of CTCs (1-10 cells/mL). In this work, a plasmonic titled fiber Bragg grating biosensor is proposed for detection of breast cancer cells. The biosensor is made by an 18° TFBG with a 50 nm-thick gold nanofilm coating over the surface of the fiber, further immobilized with a specific antibody against GPR30, which is a membrane receptor expressed in many breast cancers, serving as bait. In vitro tests have confirmed that the proposed biosensor can detect breast cancer cells in concentration of 5 cells/mL within 20 minutes and has good linearity in the range of 5–1000 cells/mL, which has met the requirement of CTC detection in real conditions. Furthermore, theoretical analysis based on the experimental results shows that the limit of detection can even reach single-cell level. Our proposed biosensor has a simple structure, is easy to manufacture, is of small size, and has a good performance, making it a good choice for real-time, label-free, and milliliter-volume detection of cancer cells in future.

Funder

Basic and Applied Basic Research Foundation of Guangdong Province

2020 LKSF Cross-Disciplinary Research Projects

Key Project of Basic Research and Applied Basic Research in Ordinary Universities of Guangdong Province

Publisher

Optica Publishing Group

Subject

Atomic and Molecular Physics, and Optics,Biotechnology

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