Affiliation:
1. Southern University of Science and Technology
2. Zhejiang University
Abstract
Point spread function (PSF) engineering is an important technique to
encode the properties (e.g., 3D positions, color, and orientation) of
a single molecule in the shape of the PSF, often with the help of a
programmable phase modulator. A deformable mirror (DM) is currently
the most widely used phase modulator for fluorescence detection as it
shows negligible photon loss. However, it relies on careful
calibration for precise wavefront control. Therefore, design of an
optimal PSF not only relies on the theoretical calculation of the
maximum information content, but also the physical behavior of the
phase modulator, which is often ignored during the optimization
process. Here, we develop a framework for PSF engineering which could
generate a device specific optimal PSF for 3D super-resolution imaging
using a DM. We use our method to generate two types of PSFs with
depths of field comparable to the widely used astigmatism and tetrapod
PSFs, respectively. We demonstrate the superior performance of the DM
specific optimal PSF over the conventional astigmatism and tetrapod
PSF both theoretically and experimentally.
Funder
Shandong Key Research and Development
Program
Science, Technology and Innovation
Commission of Shenzhen Municipality
Guangdong Natural Science Foundation
Joint Fund
Guangdong Provincial Key Laboratory of
Advanced Biomaterials
Startup grant from Southern University of
Science and Technology
Subject
Atomic and Molecular Physics, and Optics
Cited by
10 articles.
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