Multimodal analysis of traction forces and the temperature dynamics of living cells with a diamond-embedded substrate

Author:

Kołodziej Tomasz1ORCID,Mrózek MariuszORCID,Sengottuvel Saravanan2ORCID,Głowacki Maciej J.3ORCID,Ficek Mateusz3ORCID,Gawlik WojciechORCID,Rajfur ZenonORCID,Wojciechowski Adam M.ORCID

Affiliation:

1. Jagiellonian University Medical School

2. Jagiellonian University

3. Gdansk University of Technology

Abstract

Cells and tissues are constantly exposed to chemical and physical signals that regulate physiological and pathological processes. This study explores the integration of two biophysical methods: traction force microscopy (TFM) and optically detected magnetic resonance (ODMR) to concurrently assess cellular traction forces and the local relative temperature. We present a novel elastic substrate with embedded nitrogen-vacancy microdiamonds that facilitate ODMR-TFM measurements. Optimization efforts focused on minimizing sample illumination and experiment duration to mitigate biological perturbations. Our hybrid ODMR-TFM technique yields TFM maps and achieves approximately 1 K precision in relative temperature measurements. Our setup employs a simple wide-field fluorescence microscope with standard components, demonstrating the feasibility of the proposed technique in life science laboratories. By elucidating the physical aspects of cellular behavior beyond the existing methods, this approach opens avenues for a deeper understanding of cellular processes and may inspire the development of diverse biomedical applications.

Funder

Fundacja na rzecz Nauki Polskiej

European Regional Development Fund

Publisher

Optica Publishing Group

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