Affiliation:
1. Beihang University
2. Key Laboratory of Precision Opto-Mechatronics Technology (Ministry of Education),
3. Beijing Transcend Vivoscope Biotech Co.,
4. Hangzhou Innovation Institute of Beihang University
5. Peking University
Abstract
Two-photon microscopy (TPM) based on two-dimensional micro-electro-mechanical (MEMS) system mirrors shows promising applications in biomedicine and the life sciences. To improve the imaging quality and real-time performance of TPM, this paper proposes Lissajous scanning control and image reconstruction under a feed-forward control strategy, a dual-parameter alternating drive control algorithm and segmented phase synchronization mechanism, and pipe-lined fusion-mean filtering and median filtering to suppress image noise. A 10 fps frame rate (512 × 512 pixels), a 140 µm × 140 µm field of view, and a 0.62 µm lateral resolution were achieved. The imaging capability of MEMS-based Lissajous scanning TPM was verified by ex vivo and in vivo biological tissue imaging.
Funder
National Key Research and Development Program of China
National Natural Science Foundation of China
Academic Excellence Foundation of BUAA for PHD Students
Subject
Atomic and Molecular Physics, and Optics