Effectiveness of collagen cross-linking induced by two-photon absorption properties of a femtosecond laser in ex vivo human corneal stroma-Reference-Cited by-同舟云学术

Effectiveness of collagen cross-linking induced by two-photon absorption properties of a femtosecond laser in ex vivo human corneal stroma

Published:2022-09-01 Issue:9 Volume:13 Page:5067
ISSN:2156-7085
Container-title:Biomedical Optics Express
language:en
Short-container-title:Biomed. Opt. Express

Author:

Chang Le¹,Zhang Lin¹²,Cheng Zhenzhou³⁴,Zhang Nan³⁴,Wang Congzheng⁵,Wang Yan¹²^ORCID,Liu Weiwei³⁴^ORCID

Affiliation:

1. Tianjin Medical University

2. Nankai University Affiliated Eye Hospital, Nankai University

3. Nankai University

4. Tianjin Key Laboratory of Micro-scale Optical Information Science and Technology

5. Tianjin University

Abstract

This study aimed to investigate the effectiveness of two-photon induced collagen cross-linking (CXL) using femtosecond lasers in human corneal stroma. An 800-nm femtosecond laser optical path for CXL was established. Corneal samples that received two-photon induced CXL and ultraviolet-A (UVA) CXL underwent uniaxial stretching experiments, proteolytic resistance assays and observation of collagen fiber structure changes. Two-photon induced CXL can achieve corneal stiffening effects comparable to UVA CXL and showed better advantages at low strains. The cornea after two-photon induced CXL exhibited high enzymatic resistance and tight collagen fiber arrangement. Two-photon induced CXL promises to be a new option for keratoconus.

Funder

National Natural Science Foundation of China

Publisher

Optica Publishing Group

Subject

Atomic and Molecular Physics, and Optics,Biotechnology

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