Affiliation:
1. Shanghai Deyu Intelligent Technology Co., Ltd.
2. University of California
Abstract
The oblique-incidence reflectivity difference (OI-RD) microscope is a label-free detection system for microarrays that has many successful applications in high throughput drug screening. The increase and optimization of the detection speed of the OI-RD microscope will enable it to be a potential ultra-high throughput screening tool. This work presents a series of optimization methods that can significantly reduce the time to scan an OI-RD image. The wait time for the lock-in amplifier was decreased by the proper selection of the time constant and development of a new electronic amplifier. In addition, the time for the software to acquire data and for translation stage movement was also minimized. As a result, the detection speed of the OI-RD microscope is 10 times faster than before, making the OI-RD microscope suitable for ultra-high throughput screening applications.
Funder
Pioneering Project of Academy for Engineering and Technology, Fudan University
Medical Engineering Fund of Fudan University
Shanghai Engineering Technology Research Center of Hair Medicine
Shanghai Key Discipline Construction Plan
Science and Technology Research Program of Shanghai
National Key Research and Development Program of China
Natural Science Foundation of Shanghai
National Natural Science Foundation of China
Science and Technology Commission of Shanghai Municipality
Subject
Atomic and Molecular Physics, and Optics,Biotechnology