Cellular structural and functional imaging of donor and pathological corneas with label-free dual-mode full-field optical coherence tomography

Author:

Fei Keyi,Luo Zhongzhou,Chen Yupei,Huang Yuancong,Li Saiqun,Mazlin Viacheslav1ORCID,Boccara Albert Claude1,Yuan Jin,Xiao PengORCID

Affiliation:

1. PSL Research University

Abstract

In this study, a dual-mode full-field optical coherence tomography (FFOCT) was customized for label-free static and dynamic imaging of corneal tissues, including donor grafts and pathological specimens. Static images effectively depict relatively stable structures such as stroma, scar, and nerve fibers, while dynamic images highlight cells with active intracellular metabolism, specifically for corneal epithelial cells. The dual-mode images complementarily demonstrate the 3D microstructural features of the cornea and limbus. Dual-modal imaging reveals morphological and functional changes in corneal epithelial cells without labeling, indicating cellular apoptosis, swelling, deformation, dynamic signal alterations, and distinctive features of inflammatory cells in keratoconus and corneal leukoplakia. These findings propose dual-mode FFOCT as a promising technique for cellular-level cornea and limbus imaging.

Funder

National Natural Science Foundation of China

Guangdong Provincial Department of Science and Technology

Basic and Applied Basic Research Foundation of Guangdong Province

Fundamental Research Funds of the State Key Laboratory of Ophthalmology

Publisher

Optica Publishing Group

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