Affiliation:
1. University of Shanghai for Science and Technology
Abstract
In vivo near infrared (NIR) fluorescence imaging and laser speckle
contrast imaging (LSCI) are emerging optical bioimaging modalities,
which can provide information on blood vessels morphology, volume and
the blood flow velocity. Optical tissue clearing (OTC) technique
addresses a light scattering problem in optical bioimaging, which is
imperative for the transcranial brain imaging. Herein, we report an
approach combining NIR fluorescence and LSC microscopy imaging with
OTC. A liposomal nanoformulation comprising NIR fluorescent dye ICG
and photosensitizer BPD was synthesized and injected intravenously
into mouse with OTC treated skull. Transcranial excitation of BPD in
nanoliposomes resulted in the localized, irradiation dose dependent
photodynamic damage of the brain blood vessels, which was manifested
both in NIR fluorescence and LSC transcranial imaging, revealing
changes in the vessels morphology, volume and the blood flow rate. The
developed approach allows for bimodal imaging guided, localized
vascular PDT of cancer and other diseases.
Funder
Shenzhen Science and Technology
Program
Shenzhen Key Laboratory of Photonics and
Biophotonics
National Natural Science Foundation of
China
Subject
Atomic and Molecular Physics, and Optics,Biotechnology