Affiliation:
1. Jiaxing Research Institute Zhejiang University
2. Zhejiang Lab, Research Center for Intelligent Chips and Devices
3. ZJU-Hangzhou Global Scientific and Technological Innovation Center
Abstract
Structured illumination microscopy (SIM) allows non-invasive visualization of nanoscale subcellular structures. However, image acquisition and reconstruction become the bottleneck to further improve the imaging speed. Here, we propose a method to accelerate SIM imaging by combining the spatial re-modulation principle with Fourier domain filtering and using measured illumination patterns. This approach enables high-speed, high-quality imaging of dense subcellular structures using a conventional nine-frame SIM modality without phase estimation of the patterns. In addition, seven-frame SIM reconstruction and additional hardware acceleration further improve the imaging speed using our method. Furthermore, our method is also applicable to other spatially uncorrelated illumination patterns, such as distorted sinusoidal, multifocal, and speckle patterns.
Funder
National Key R&D Program of China
“Leading Goose” R&D Program of Zhejiang
Fundamental Research Funds for the Central Universities
National Natural Science Foundation of China
Zhejiang Provincial Natural Science Foundation
Subject
Atomic and Molecular Physics, and Optics
Cited by
3 articles.
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