Method for determination of optical rotatory dispersion curve by using a polarization axis finder

Abstract

We report a new, to the best of our knowledge, measurement method of optical rotatory dispersion (ORD) based on a polarization axis finder (PAF) and digital image processing. The collimated quasi-monochromatic light is output by a monochromator and passes through a polarizer and the chiral samples, and a PAF is employed to modulate the light beam spatially to form an “hourglass” intensity pattern, which is utilized to determine the polarization direction of linearly polarized light. After being recorded by a digital camera, the modulated patterns with and without samples are analyzed by our specially designed digital image processing program to calculate the optical rotation (OR) angle of the chiral substance at a certain wavelength, and the ORD curve can be obtained by fitting a series of OR angles with different wavelengths. The ORD curves of two standard quartz plates with opposite chirality and a glucose solution with different concentrations are measured experimentally, and the fitted coefficients and measurement results agree well with their theoretical values, which indicates that the presented method is an effective and accurate way to determine the ORD of chiral substances conveniently.