Tunable liquid lens for three-photon excitation microscopy

Author:

Gilinsky Samuel D.,Jung Diane N.ORCID,Futia Greg L.1ORCID,Zohrabi Mo,Welton Tarah A.1,Supekar Omkar D.ORCID,Gibson Emily A.1,Restrepo Diego1,Bright Victor M.,Gopinath Juliet T.ORCID

Affiliation:

1. University of Colorado Anschutz Medical Campus

Abstract

We demonstrate a novel electrowetting liquid combination using a room temperature ionic liquid (RTIL) and a nonpolar liquid, 1-phenyl-1-cyclohexene (PCH) suitable for focus-tunable 3-photon microscopy. We show that both liquids have over 90% transmission at 1300 nm over a 1.1 mm pathlength and an index of refraction contrast of 0.123. A lens using these liquids can be tuned from a contact angle of 133 to 48° with applied voltages of 0 and 60 V, respectively. Finally, a three-photon imaging system including an RTIL electrowetting lens was used to image a mouse brain slice. Axial scans taken with an electrowetting lens show excellent agreement with images acquired using a mechanically scanned objective.

Funder

National Science Foundation

National Institutes of Health

Office of Naval Research

University of Colorado

Publisher

Optica Publishing Group

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