Affiliation:
1. Heriot-Watt University
Abstract
In this work a combined fluorescence lifetime and surface topographical
imaging system is demonstrated. Based around a 126 × 192 time resolved
single photon avalanche diode (SPAD) array operating in time
correlated single-photon counting (TCSPC) mode, both the fluorescence
lifetime and time of flight (ToF) can be calculated on a pixel by
pixel basis. Initial tests on fluorescent samples show it is able to
provide 4 mm resolution in distance and 0.4 ns resolution in lifetime.
This combined modality has potential biomedical applications such as
surgical guidance, endoscopy, and diagnostic imaging. The system is
demonstrated on both ovine and human pulmonary tissue samples, where
it offers excellent fluorescence lifetime contrast whilst also giving
a measure of the distance to the sample surface.
Funder
Engineering and Physical Sciences
Research Council
Subject
Atomic and Molecular Physics, and Optics,Biotechnology