Adaptive optics scanning laser ophthalmoscopy and optical coherence tomography (AO-SLO-OCT) system for in vivo mouse retina imaging

Author:

Zhang Pengfei1ORCID,Wahl Daniel J.2,Mocci Jacopo3ORCID,Miller Eric B.4,Bonora Stefano5,Sarunic Marinko V.26,Zawadzki Robert J.7ORCID

Affiliation:

1. Dalian University of Technology

2. Simon Fraser University

3. Dynamic Optics srl

4. University of California

5. CNR-Institute for Photonics and Nanotechnology

6. University College London

7. University of California Davis

Abstract

Optical coherence tomography (OCT) and scanning laser ophthalmoscopy (SLO) are imaging technologies invented in the 1980s that have revolutionized the field of in vivo retinal diagnostics and are now commonly used in ophthalmology clinics as well as in vision science research. Adaptive optics (AO) technology enables high-fidelity correction of ocular aberrations, resulting in improved resolution and sensitivity for both SLO and OCT systems. The potential of gathering multi-modal cellular-resolution information in a single instrument is of great interest to the ophthalmic imaging community. Although similar instruments have been developed for imaging the human retina, developing such a system for mice will benefit basic science research and should help with further dissemination of AO technology. Here, we present our work integrating OCT into an existing mouse retinal AO-SLO system, resulting in a multi-modal AO-enhanced imaging system of the living mouse eye. The new system allows either independent or simultaneous data acquisition of AO-SLO and AO-OCT, depending on the requirements of specific scientific experiments. The system allows a data acquisition speed of 200 kHz A-scans/pixel rate for OCT and SLO, respectively. It offers ∼6 µm axial resolution for AO-OCT and a ∼1 µm lateral resolution for AO-SLO-OCT imaging.

Funder

National Science Foundation

National Eye Institute

Dalian University of Technology

National Natural Science Foundation of China

Canadian Institutes of Health Research

Publisher

Optica Publishing Group

Subject

Atomic and Molecular Physics, and Optics,Biotechnology

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