Author:
Costantini Irene,Di Meo Danila,Ramazzotti Josephine,Cheli Franco,Sorelli Michele,Mazzamuto Giacomo,Checcucci Curzio,Frasconi Paolo,Pavone Francesco Saverio
Abstract
Using the SHORT clearing method in combination with an advanced double-view light-sheet fluorescence microscope and an automated machine-learning-based tool we performed 3D reconstruction of a whole human Broca’s area at micrometer resolution.