Label-Free Tracking of Myelin Dynamics in Subcortical White Matter of a Mouse Model of Multiple Sclerosis using Third Harmonic Generation Microscopy

Abstract

Third harmonic generation with 1320-nm, femtosecond pulses can visualize individual myelinated axons in subcortical white matter through intact cortex of live mice. In a cuprizone multiple sclerosis model, this enabled longitudinal tracking of myelin damage.