Affiliation:
1. The University of Western Australia
2. Australian Research Council Centre for Personalised Therapeutics Technologies
3. Harry Perkins Institute of Medical Research
Abstract
The importance of cellular-scale mechanical properties is
well-established, yet it is challenging to map subcellular elasticity
in three dimensions. We present subcellular mechano-microscopy, an
optical coherence microscopy (OCM)-based variant of three-dimensional
(3-D) compression optical coherence elastography (OCE) that provides
an elasticity system resolution of 5 × 5 × 5 µm: a 7-fold improvement
in system resolution over previous OCE studies of cells. The improved
resolution is achieved through a ∼5-fold improvement in optical
resolution, refinement of the strain estimation algorithm, and
demonstration that mechanical deformation of subcellular features
provides feature resolution far greater than that demonstrated
previously on larger features with diameter >250 µm. We use
mechano-microscopy to image adipose-derived stem cells encapsulated in
gelatin methacryloyl. We compare our results with compression OCE and
demonstrate that mechano-microscopy can provide contrast from
subcellular features not visible using OCE.
Funder
Australian Research Council
Department of Health, Australian
Government
Cancer Council Western
Australia
National Heart Foundation of
Australia
Department of Jobs, Tourism, Science and
Innovation, Government of Western Australia
Subject
Atomic and Molecular Physics, and Optics
Cited by
11 articles.
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