Research on partially coherent spatial light interference microscopy

Abstract

Based on partial coherence theory, this study rigorously deduces the principle of spatial light interference microscopy (SLIM) and improves the calculation method of SLIM. The main problem we found with SLIM is that it simply defaults the phase of the direct light to 0. To address this problem, we propose and experimentally demonstrate a double four-step phase shift method. Simulation results show that this method can reduce the relative error of oil-immersed microsphere reconstruction to about 3.7%, and for red blood cell reconstruction, the relative error can be reduced to about 13%.