Affiliation:
1. Universidad de Concepción
Abstract
A three-dimensional point spread function experimental estimation method based on the system’s focal plane array spatial local impulse response of a mid-wave infrared microscope is presented. The method uses several out-of-focus two-dimensional point spread function planes to achieve a single three-dimensional point spread function of the whole microscope’s optical spreading, expanding the limits of infrared optical technology by one dimension. This technique includes stages of image acquisition, nonuniformity correction, filtering, and multi-planar reconstruction steps, and its effectiveness is demonstrated on biological sample image restoration by means of a multi-planar refocusing application.
Funder
Agencia Nacional de Investigación y Desarrollo
Fondo Nacional de Desarrollo Científico y Tecnológico
Subject
Atomic and Molecular Physics, and Optics,Engineering (miscellaneous),Electrical and Electronic Engineering