Deep-UV Surface Plasmon for Bio-Imaging

Abstract

Surface plasmon resonance (SPR) has attracted considerable attention in relation to optical biosensors, solar cells, holography, and other applications, because it can enhance the electric field of incident light by several factors of ten.12 Its application to bioimaging has also been extensively investigated. Fluorescence imaging has been widely used to analyze the dynamic behavior of cellular components. Recently, deep-ultraviolet surface plasmon resonance (DUV-SPR) has also been investigated [1-3]. It can potentially be used in combination with photoelectron emission and Raman scattering measurements because of the high photon energy associated with DUV light. In the present study, DUV-SPR was used for the simultaneous excitation of enhanced multicolor fluorescence from several types of quantum dots. This technique was also applied to high-sensitivity observation of dye-labeled cells.