Author:
Kubo Toshiki,Temma Kenta,Sugiura Kazunori,Shinoda Hajime,Lu Kai,Smith Nicholas I.,Matsuda Tomoki,Nagai Takeharu,Fujita Katsumasa
Abstract
We experimentally demonstrated the photo-activation of reversibly photo-switchable fluorescent proteins by visible-wavelength two-photon excitation and applied the activation technique in confocal imaging of biological cells. Higher spatial resolution than conventional confocal microscopy was confirmed.