Fourier Transform Infrared Microscopy Based Differentiation Between Healthy and Infected Cerebrospinal Fluid (CSF) in Meningitis

Abstract

We have projected Fourier Transform Infrared Spectroscopy (FTIR) for differentiation of normal CSF from bacterial, fungal and viral meningitis CSF. Changes were observed in polysaccharide region (1119/ 1142 cm-1) in bacterial and fungal meningitis indicating signals from ultrastructure of streptococcus pneumoniae and candida albicans. In viral meningitis, phospholipids have shown major changes at 1443 cm-1. Also, Amide III decreased in diseased states while Amide I was significantly raised when compared to normal CSF.